store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
NU 9056 是一种有效且选择性的 KAT5 组蛋白乙酰转移酶抑制剂,IC50 为 2 µM。 NU 9056 阻断 DNA 损伤反应并抑制前列腺癌细胞系中的蛋白质乙酰化。
产品描述 | NU 9056 is an effective and selective inhibitor of KAT5 histone acetyltransferase with an IC50 of 2 µM. NU 9056 blocks DNA damage response and inhibits protein acetylation in prostate cancer cell lines. |
体外活性 | NU 9056 shows IC50s of 60, 36, and >100 μM for KAT5, p300, pCAF and GCN5, respectively. In LNCaP cells, NU 9056 (17 µM, 24 µM, 36 µM) activates both caspase 3 and caspase 9 in a time- and concentration-dependent manner thereby inducing apoptosis. NU 9056 (2.5 µM, 5 µM, 10 µM, 20 µM, 40 µM) decreases the levels of acetylated histone H4K16, H3K14 and H4K8. NU 9056 causes a reduction of androgen receptor, prostate specific antigen, p53 and p21 protein levels[2]. |
体内活性 | In mice, NU 9056 (2 μg/g) inhibits Tip60 and reduces H2A.Z binding at the the +1 nucleosome of Arc and Syp and the -1 nucleosome of Arc. NU 9056 increases the acetylation at the +1 nucleosome of Gria4 and the -1 nucleosome of Fos, Tacstd2, and Gria4[1]. |
别名 | NU9056 |
分子量 | 232.37 |
分子式 | C6H4N2S4 |
CAS No. | 1450644-28-6 |
store at low temperature | Powder: -20°C for 3 years | In solvent: -80°C for 1 year
DMSO: 112.5 mg/mL (484.1 mM), Sonication is recommended.
可选溶剂 | 浓度 体积 质量 | 1 mg | 5 mg | 10 mg | 25 mg |
DMSO | 1 mM | 4.3035 mL | 21.5174 mL | 43.0348 mL | 107.587 mL |
5 mM | 0.8607 mL | 4.3035 mL | 8.607 mL | 21.5174 mL | |
10 mM | 0.4303 mL | 2.1517 mL | 4.3035 mL | 10.7587 mL | |
20 mM | 0.2152 mL | 1.0759 mL | 2.1517 mL | 5.3794 mL | |
50 mM | 0.0861 mL | 0.4303 mL | 0.8607 mL | 2.1517 mL | |
100 mM | 0.043 mL | 0.2152 mL | 0.4303 mL | 1.0759 mL |
对于不同动物的给药剂量换算,您也可以参考 更多...
请在以下方框中输入您的动物实验信息后点击计算,可以得到母液配置方法和体内配方的制备方法: 比如您的给药剂量是10 mg/kg,每只动物体重20 g,给药体积100 μL,一共给药动物10 只,您使用的配方为5% DMSO+30% PEG300+5% Tween 80+60% ddH2O。那么您的工作液浓度为2 mg/mL。
母液配置方法:2 mg 药物溶于 50 μL DMSO (母液浓度为 40 mg/mL), 如您需要配置的浓度超过该产品的溶解度,请先与我们联系。
体内配方的制备方法:取 50 μL DMSO 主液,加入 300 μL PEG300, 混匀澄清,再加 50 μL Tween 80,混匀澄清,再加 600 μL ddH2O, 混匀澄清。
您可能有的问题的答案可以在抑制剂处理说明中找到,包括如何准备库存溶液,如何存储产品,以及基于细胞的分析和动物实验需要特别注意的问题。
NU 9056 1450644-28-6 Chromatin/Epigenetic Histone Acetyltransferase NU-9056 NU9056 Inhibitor inhibitor inhibit